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  1. 国際農研研究成果
  2. 学術雑誌論文

Cryopreservation of spermatogonia of the giant freshwater prawn, Macrobrachium rosenbergii, using slow and ultra-rapid freezing

https://jircas.repo.nii.ac.jp/records/2000884
https://jircas.repo.nii.ac.jp/records/2000884
602eacd2-d069-4900-9ce8-1bb6fd0d8d09
名前 / ファイル ライセンス アクション
2000884.pdf 2000884 (5.3 MB)
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Item type 国際農研デフォルトアイテムタイプ(フル)(1)
公開日 2025-05-26
タイトル
タイトル Cryopreservation of spermatogonia of the giant freshwater prawn, Macrobrachium rosenbergii, using slow and ultra-rapid freezing
言語 en
作成者 Okutsu, Tomoyuki

× Okutsu, Tomoyuki

ORCID 0009-0003-7607-7848

en Okutsu, Tomoyuki
ISNI Japan International Research Center for Agricultural Sciences 0000000121078171

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Rakbanjong, Natthida

× Rakbanjong, Natthida

en Rakbanjong, Natthida
ISNI Prince of Songkla University 0000000404701162

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Shikina, Shinya

× Shikina, Shinya

en Shikina, Shinya
ISNI National Taiwan Ocean University 0000000103133026

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Miwa, Misako

× Miwa, Misako

en Miwa, Misako
ISNI Tokyo University of Marine Science and Technology 0000000106956482

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Wonglapsuwan, Monwadee

× Wonglapsuwan, Monwadee

ORCID 0000-0002-9272-684X

en Wonglapsuwan, Monwadee
ISNI Prince of Songkla University 0000000404701162

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 © 2025 The Authors. Published by Elsevier Inc. on behalf of Society for Cryobiology.
権利情報
権利情報Resource http://creativecommons.org/licenses/by-nc-nd/4.0/
権利情報 This is an open access article under the CC BY-NC-ND license
主題
主題 Aquaculture, Crustacean, Cryoprotectant, Shrimp, Spermatogonia
内容記述
内容記述タイプ Other
内容記述 The giant freshwater prawn, Macrobrachium rosenbergii, is an important species that is widely raised in tropical and subtropical regions. To develop techniques for preserving valuable traits in this species, we have devised protocols for cryopreserving spermatogonia—progenitor cells that give rise to spermatozoa through spermatogenesis— using slow freezing and ultra-rapid freezing techniques. Optimization of cryoprotectants and their concentrations revealed that 10% dimethyl sulfoxide (ME2SO) is effective as a cryoprotectant in both methods. The optimal time for equilibration to the cryoprotectant before slow freezing was determined to be 15 min. Thawing temperature had a significant effect on cell recovery and viability, both being markedly higher when cells were thawed at 10℃ than at 27℃. After long-term storage in liquid nitrogen, cells preserved by ultra-rapid freezing exhibited higher recovery and viability rates than those preserved using the slow freezing method, with recovery rates of up to 86.4% ± 5.9% at 6 months. Therefore, using 10% ME2SO with the ultra-rapid freezing method is recommended as the optimal protocol for long-term cryopreservation of M. rosenbergii spermatogonia.
言語 en
出版者
出版者 Elsevier
日付
日付 2025-03-31
日付タイプ Accepted
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.cryobiol.2025.105242
収録物識別子
収録物識別子タイプ PISSN
収録物識別子 0011-2240
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA00620601
書誌情報 en : Cryobiology

巻 119, p. 105242, 発行日 2025-04-15
助成情報
識別子タイプ Crossref Funder
助成機関識別子 https://doi.org/10.13039/501100002241
助成機関名 Japan Science and Technology Agency (JST)(en)
研究課題番号URI https://doi.org/10.52926/JPMJSA1806
研究課題番号 JPMJSA1806
研究課題名 Science and Technology Research Partnership for Sustainable Development (SATREPS) : Utilization of Thailand Local Genetic Resources to Develop Novel Farmed Fish for the Global Market(en)
助成情報
助成機関名 National Science, Research and Innovation Fund (NSRF)(en)
研究課題番号 SCI6505113a
助成情報
識別子タイプ Crossref Funder
助成機関識別子 https://doi.org/10.13039/501100004508
助成機関名 Prince of Songkla University(en)
研究課題番号 SCI6505113a
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