| Item type |
国際農研デフォルトアイテムタイプ（フル）(1) |
| 公開日 |
2025-05-26 |
| タイトル |
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タイトル |
Cryopreservation of spermatogonia of the giant freshwater prawn, Macrobrachium rosenbergii, using slow and ultra-rapid freezing |
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言語 |
en |
| 作成者 |
Okutsu, Tomoyuki
Rakbanjong, Natthida
Shikina, Shinya
Miwa, Misako
Wonglapsuwan, Monwadee
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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権利情報 |
© 2025 The Authors. Published by Elsevier Inc. on behalf of Society for Cryobiology. |
| 権利情報 |
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|
権利情報Resource |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
This is an open access article under the CC BY-NC-ND license |
| 主題 |
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主題 |
Aquaculture, Crustacean, Cryoprotectant, Shrimp, Spermatogonia |
| 内容記述 |
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内容記述タイプ |
Other |
|
内容記述 |
The giant freshwater prawn, Macrobrachium rosenbergii, is an important species that is widely raised in tropical and subtropical regions. To develop techniques for preserving valuable traits in this species, we have devised protocols for cryopreserving spermatogonia—progenitor cells that give rise to spermatozoa through spermatogenesis— using slow freezing and ultra-rapid freezing techniques. Optimization of cryoprotectants and their concentrations revealed that 10% dimethyl sulfoxide (ME2SO) is effective as a cryoprotectant in both methods. The optimal time for equilibration to the cryoprotectant before slow freezing was determined to be 15 min. Thawing temperature had a significant effect on cell recovery and viability, both being markedly higher when cells were thawed at 10℃ than at 27℃. After long-term storage in liquid nitrogen, cells preserved by ultra-rapid freezing exhibited higher recovery and viability rates than those preserved using the slow freezing method, with recovery rates of up to 86.4% ± 5.9% at 6 months. Therefore, using 10% ME2SO with the ultra-rapid freezing method is recommended as the optimal protocol for long-term cryopreservation of M. rosenbergii spermatogonia. |
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言語 |
en |
| 出版者 |
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出版者 |
Elsevier |
| 日付 |
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日付 |
2025-03-31 |
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日付タイプ |
Accepted |
| 言語 |
|
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1016/j.cryobiol.2025.105242 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0011-2240 |
| 収録物識別子 |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00620601 |
| 書誌情報 |
en : Cryobiology
巻 119,
p. 105242,
発行日 2025-04-15
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| 助成情報 |
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識別子タイプ |
Crossref Funder |
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助成機関識別子 |
https://doi.org/10.13039/501100002241 |
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助成機関名 |
Japan Science and Technology Agency (JST)(en) |
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研究課題番号URI |
https://doi.org/10.52926/JPMJSA1806 |
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|
研究課題番号 |
JPMJSA1806 |
|
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研究課題名 |
Science and Technology Research Partnership for Sustainable Development (SATREPS) : Utilization of Thailand Local Genetic Resources to Develop Novel Farmed Fish for the Global Market(en) |
| 助成情報 |
|
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助成機関名 |
National Science, Research and Innovation Fund (NSRF)(en) |
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研究課題番号 |
SCI6505113a |
| 助成情報 |
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識別子タイプ |
Crossref Funder |
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助成機関識別子 |
https://doi.org/10.13039/501100004508 |
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助成機関名 |
Prince of Songkla University(en) |
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研究課題番号 |
SCI6505113a |
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