{"created":"2025-05-22T06:05:10.762700+00:00","id":2000884,"links":{},"metadata":{"_buckets":{"deposit":"ed4c2893-d532-45f1-9d28-772cfb3a0be3"},"_deposit":{"created_by":74,"id":"2000884","owners":[74],"pid":{"revision_id":0,"type":"depid","value":"2000884"},"status":"published"},"_oai":{"id":"oai:jircas.repo.nii.ac.jp:02000884","sets":["1718256554766:1718256617194"]},"author_link":[],"control_number":"2000884","item_30002_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-04-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"105242","bibliographicVolumeNumber":"119","bibliographic_titles":[{"bibliographic_title":"Cryobiology","bibliographic_titleLang":"en"}]}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000121078171","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000121078171"}],"affiliationNames":[{"affiliationName":"Japan International Research Center for Agricultural Sciences","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Okutsu, Tomoyuki","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author","nameIdentifiers":[{"nameIdentifier":"0009-0003-7607-7848","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0009-0003-7607-7848"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000404701162","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000404701162"}],"affiliationNames":[{"affiliationName":"Prince of Songkla University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Rakbanjong, Natthida","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000103133026","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000103133026"}],"affiliationNames":[{"affiliationName":"National Taiwan Ocean University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Shikina, Shinya","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106956482","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106956482"}],"affiliationNames":[{"affiliationName":"Tokyo University of Marine Science and Technology","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Miwa, Misako","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000404701162","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000404701162"}],"affiliationNames":[{"affiliationName":"Prince of Songkla University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Wonglapsuwan, Monwadee","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author","nameIdentifiers":[{"nameIdentifier":"0000-0002-9272-684X","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-9272-684X"}]}]},"item_30002_date11":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2025-03-31","subitem_date_issued_type":"Accepted"}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The giant freshwater prawn, Macrobrachium rosenbergii, is an important species that is widely raised in tropical and subtropical regions. To develop techniques for preserving valuable traits in this species, we have devised protocols for cryopreserving spermatogonia—progenitor cells that give rise to spermatozoa through spermatogenesis— using slow freezing and ultra-rapid freezing techniques. Optimization of cryoprotectants and their concentrations revealed that 10% dimethyl sulfoxide (ME2SO) is effective as a cryoprotectant in both methods. The optimal time for equilibration to the cryoprotectant before slow freezing was determined to be 15 min. Thawing temperature had a significant effect on cell recovery and viability, both being markedly higher when cells were thawed at 10℃ than at 27℃. After long-term storage in liquid nitrogen, cells preserved by ultra-rapid freezing exhibited higher recovery and viability rates than those preserved using the slow freezing method, with recovery rates of up to 86.4% ± 5.9% at 6 months. Therefore, using 10% ME2SO with the ultra-rapid freezing method is recommended as the optimal protocol for long-term cryopreservation of M. rosenbergii spermatogonia.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-05-26"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2025-04-15"}],"filename":"2000884.pdf","filesize":[{"value":"5.3 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"label":"2000884","objectType":"fulltext","url":"https://jircas.repo.nii.ac.jp/record/2000884/files/2000884.pdf"},"version_id":"ca624a1f-d2dd-4cfb-bb7f-cba414f92f7e"}]},"item_30002_funding_reference21":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"JPMJSA1806","subitem_award_uri":"https://doi.org/10.52926/JPMJSA1806"},"subitem_award_titles":[{"subitem_award_title":"Science and Technology Research Partnership for Sustainable Development (SATREPS) : Utilization of Thailand Local Genetic Resources to Develop Novel Farmed Fish for the Global Market","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100002241","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Japan Science and Technology Agency (JST)","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"SCI6505113a"},"subitem_funder_names":[{"subitem_funder_name":"National Science, Research and Innovation Fund (NSRF)","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"SCI6505113a"},"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100004508","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Prince of Songkla University","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"a1B4","subitem_award_uri":"https://www.jircas.go.jp/en/program/prob/b4"},"subitem_award_titles":[{"subitem_award_title":"Ecosystem approach to aquaculture","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100009472","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Japan International Research Center for Agricultural Sciences (JIRCAS)","subitem_funder_name_language":"en"}]}]},"item_30002_language12":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_30002_relation18":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.cryobiol.2025.105242","subitem_relation_type_select":"DOI"}}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_30002_rights6":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2025 The Authors. Published by Elsevier Inc. on behalf of Society for Cryobiology.","subitem_rights_language":"en"},{"subitem_rights":"This is an open access article under the CC BY-NC-ND license","subitem_rights_language":"en","subitem_rights_resource":"http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_30002_source_identifier22":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0011-2240","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA00620601","subitem_source_identifier_type":"NCID"}]},"item_30002_subject8":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"Aquaculture","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Crustacean","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryoprotectant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Shrimp","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Spermatogonia","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cryopreservation of spermatogonia of the giant freshwater prawn, Macrobrachium rosenbergii, using slow and ultra-rapid freezing","subitem_title_language":"en"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"Cryopreservation of spermatogonia of the giant freshwater prawn, Macrobrachium rosenbergii, using slow and ultra-rapid freezing","item_type_id":"40039","owner":"74","path":["1718256617194"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-05-26"},"publish_date":"2025-05-26","publish_status":"0","recid":"2000884","relation_version_is_last":true,"title":["Cryopreservation of spermatogonia of the giant freshwater prawn, Macrobrachium rosenbergii, using slow and ultra-rapid freezing"],"weko_creator_id":"74","weko_shared_id":-1},"updated":"2025-06-17T04:36:25.965592+00:00"}