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Laboratory manual for studies on soybean rust resistance
https://doi.org/10.34556/0002000882
https://doi.org/10.34556/00020008829974c168-10cf-43c4-a3fa-756c3cb848b8
名前 / ファイル | ライセンス | アクション |
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Item type | 国際農研デフォルトアイテムタイプ(フル)(1) | |||||||||||||
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公開日 | 2025-05-13 | |||||||||||||
タイトル | ||||||||||||||
タイトル | Laboratory manual for studies on soybean rust resistance | |||||||||||||
言語 | en | |||||||||||||
作成者 |
Yamanaka, Naoki
× Yamanaka, Naoki
ORCID
0000-0002-5099-3157
× Kato, Masayasu
ORCID
0000-0003-3642-1934
× Akamatsu, Hajime
× Yamaoka, Yuichi
ORCID
0000-0002-0411-6110
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アクセス権 | ||||||||||||||
アクセス権 | open access | |||||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||
権利情報 | ||||||||||||||
権利情報 | © 2013-2023 Japan International Research Center for Agricultural Sciences (JIRCAS) | |||||||||||||
権利情報 | ||||||||||||||
権利情報Resource | https://www.jircas.go.jp/en/publication/manual_guideline/30 | |||||||||||||
権利情報 | About the copyright and the notice with regard to use: The copyright of this manual belongs to Japan International Research Center for Agricultural Sciences (JIRCAS). This manual may not be modified or copied in whole or in part without the permission of JIRCAS (headquarters: 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan), except for brief excerpts in connection with reviews or academic activities. In case you make the research results based on this manual public via articles, meetings, print, Internet, etc., you must cite this manual and its URL. In addition, please contact us through this form. The experimental data in this manual were obtained by the methods in it, however, they may fluctuate according to the differences in cultivation conditions. Please be aware that the contents of the manual will be periodically updated and check the date and the version of the downloaded manual. In case you have any questions or comments regarding for this manual, please mail to us through this form. Please download the files if you confirm and agree to the above conditions. | |||||||||||||
内容記述 | ||||||||||||||
内容記述タイプ | TableOfContents | |||||||||||||
内容記述 | Chapter 1: Evaluation of resistance to soybean rust Section 1-1. Multiplication of rust urediniospores : 3 1-2. Single-lesion isolation : 6 1-2’. Single-spore isolation : 9 1-3. Inoculation of soybean with spore suspension : 12 1-3’. Inoculation of soybean with spore suspension (Leaf culture method) : 17 1-4. Evaluation of rust pathogenicity : 18 1-5. Rust resistance of soybean genotypes : 21 1-6. Rust tolerance of soybean genotypes : 24 1-7. Data analysis : 27 Chapter 2: Simple sequence repeat (SSR) marker analysis (Toward marker-assisted selection to introduce Rpp genes) 2-1. Sampling for DNA extraction : 29 2-2. DNA extraction : 32 2-3. Adjustment of DNA concentration : 34 2-4. PCR : 37 2-5. Acrylamide gel electrophoresis : 39 2-6. Data analysis : 43 Acknowledgments : 50 References : 52 Version info and authors : 55 |
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言語 | en | |||||||||||||
内容記述 | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | Version information-Publication date on the website Version 27 (English and Spanish): July 3, 2023 |
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言語 | en | |||||||||||||
出版者 | ||||||||||||||
出版者 | Japan International Research Center for Agricultural Sciences | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18gh | |||||||||||||
資源タイプ | technical report | |||||||||||||
出版タイプ | ||||||||||||||
出版タイプ | VoR | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
ID登録 | ||||||||||||||
ID登録 | 10.34556/0002000882 | |||||||||||||
ID登録タイプ | JaLC | |||||||||||||
関連情報 | ||||||||||||||
関連タイプ | hasVersion | |||||||||||||
識別子タイプ | URI | |||||||||||||
関連識別子 | https://jircas.repo.nii.ac.jp/records/2000881 | |||||||||||||
関連名称 | Manual de laboratorio para estudios sobre la resistencia a roya de la soja | |||||||||||||
書誌情報 |
p. 1-55, 発行日 2023-07-03 |
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助成情報 | ||||||||||||||
識別子タイプ | Crossref Funder | |||||||||||||
助成機関識別子 | https://doi.org/10.13039/501100009472 | |||||||||||||
助成機関名 | Japan International Research Center for Agricultural Sciences (JIRCAS)(en) | |||||||||||||
研究課題番号URI | https://www.jircas.go.jp/en/program/prob/b1 | |||||||||||||
研究課題番号 | a1B1 | |||||||||||||
研究課題名 | Development of resilient crops and production technologies(en) | |||||||||||||
助成情報 | ||||||||||||||
識別子タイプ | Crossref Funder | |||||||||||||
助成機関識別子 | https://doi.org/10.13039/501100009472 | |||||||||||||
助成機関名 | Japan International Research Center for Agricultural Sciences (JIRCAS)(en) | |||||||||||||
研究課題番号URI | https://www.jircas.go.jp/en/program/program_b/b4 | |||||||||||||
研究課題番号 | B4 | |||||||||||||
研究課題名 | Development of Management Technologies against Migrating Pests and Diseases over Countries(en) | |||||||||||||
助成情報 | ||||||||||||||
識別子タイプ | Crossref Funder | |||||||||||||
助成機関識別子 | https://doi.org/10.13039/501100009472 | |||||||||||||
助成機関名 | Japan International Research Center for Agricultural Sciences (JIRCAS)(en) | |||||||||||||
研究課題番号URI | https://www.jircas.go.jp/en/program/program_b/20161108 | |||||||||||||
研究課題番号 | B3 | |||||||||||||
研究課題名 | Development of Breeding Technologies toward Improved Production and Stable Supply of Upland Crops(en) |