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Laboratory manual for studies on soybean rust resistance

https://doi.org/10.34556/0002000882
https://doi.org/10.34556/0002000882
9974c168-10cf-43c4-a3fa-756c3cb848b8
名前 / ファイル ライセンス アクション
Laboratory_manual_v.27.1.pdf Laboratory_manual_v.27.1 (3.6 MB)
Laboratory_manual_V27.1_Thumbnail.jpg Laboratory_manual_V27.1_Thumbnail (28 KB)
Item type 国際農研デフォルトアイテムタイプ(フル)(1)
公開日 2025-05-13
タイトル
タイトル Laboratory manual for studies on soybean rust resistance
言語 en
作成者 Yamanaka, Naoki

× Yamanaka, Naoki

ORCID 0000-0002-5099-3157

en Yamanaka, Naoki
ISNI Japan International Research Center for Agricultural Sciences 0000000121078171

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Kato, Masayasu

× Kato, Masayasu

ORCID 0000-0003-3642-1934

en Kato, Masayasu
ISNI Japan International Research Center for Agricultural Sciences 0000000121078171

Search repository
Akamatsu, Hajime

× Akamatsu, Hajime

en Akamatsu, Hajime
ISNI National Agriculture and Food Research Organization 0000000122220432

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Yamaoka, Yuichi

× Yamaoka, Yuichi

ORCID 0000-0002-0411-6110

en Yamaoka, Yuichi
ISNI University of Tsukuba 0000000123694728

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 © 2013-2023 Japan International Research Center for Agricultural Sciences (JIRCAS)
権利情報
権利情報Resource https://www.jircas.go.jp/en/publication/manual_guideline/30
権利情報 About the copyright and the notice with regard to use: The copyright of this manual belongs to Japan International Research Center for Agricultural Sciences (JIRCAS). This manual may not be modified or copied in whole or in part without the permission of JIRCAS (headquarters: 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan), except for brief excerpts in connection with reviews or academic activities. In case you make the research results based on this manual public via articles, meetings, print, Internet, etc., you must cite this manual and its URL. In addition, please contact us through this form. The experimental data in this manual were obtained by the methods in it, however, they may fluctuate according to the differences in cultivation conditions. Please be aware that the contents of the manual will be periodically updated and check the date and the version of the downloaded manual. In case you have any questions or comments regarding for this manual, please mail to us through this form. Please download the files if you confirm and agree to the above conditions.
内容記述
内容記述タイプ TableOfContents
内容記述 Chapter 1: Evaluation of resistance to soybean rust Section
1-1. Multiplication of rust urediniospores : 3
1-2. Single-lesion isolation : 6
1-2’. Single-spore isolation : 9
1-3. Inoculation of soybean with spore suspension : 12
1-3’. Inoculation of soybean with spore suspension (Leaf culture method) : 17
1-4. Evaluation of rust pathogenicity : 18
1-5. Rust resistance of soybean genotypes : 21
1-6. Rust tolerance of soybean genotypes : 24
1-7. Data analysis : 27

Chapter 2: Simple sequence repeat (SSR) marker analysis (Toward marker-assisted selection to introduce Rpp genes)
2-1. Sampling for DNA extraction : 29
2-2. DNA extraction : 32
2-3. Adjustment of DNA concentration : 34
2-4. PCR : 37
2-5. Acrylamide gel electrophoresis : 39
2-6. Data analysis : 43

Acknowledgments : 50
References : 52
Version info and authors : 55
言語 en
内容記述
内容記述タイプ Other
内容記述 Version information-Publication date on the website
Version 27 (English and Spanish): July 3, 2023
言語 en
出版者
出版者 Japan International Research Center for Agricultural Sciences
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_18gh
資源タイプ technical report
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
ID登録
ID登録 10.34556/0002000882
ID登録タイプ JaLC
関連情報
関連タイプ hasVersion
識別子タイプ URI
関連識別子 https://jircas.repo.nii.ac.jp/records/2000881
関連名称 Manual de laboratorio para estudios sobre la resistencia a roya de la soja
書誌情報
p. 1-55, 発行日 2023-07-03
助成情報
識別子タイプ Crossref Funder
助成機関識別子 https://doi.org/10.13039/501100009472
助成機関名 Japan International Research Center for Agricultural Sciences (JIRCAS)(en)
研究課題番号URI https://www.jircas.go.jp/en/program/prob/b1
研究課題番号 a1B1
研究課題名 Development of resilient crops and production technologies(en)
助成情報
識別子タイプ Crossref Funder
助成機関識別子 https://doi.org/10.13039/501100009472
助成機関名 Japan International Research Center for Agricultural Sciences (JIRCAS)(en)
研究課題番号URI https://www.jircas.go.jp/en/program/program_b/b4
研究課題番号 B4
研究課題名 Development of Management Technologies against Migrating Pests and Diseases over Countries(en)
助成情報
識別子タイプ Crossref Funder
助成機関識別子 https://doi.org/10.13039/501100009472
助成機関名 Japan International Research Center for Agricultural Sciences (JIRCAS)(en)
研究課題番号URI https://www.jircas.go.jp/en/program/program_b/20161108
研究課題番号 B3
研究課題名 Development of Breeding Technologies toward Improved Production and Stable Supply of Upland Crops(en)
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