{"created":"2025-05-13T06:33:19.703486+00:00","id":2000882,"links":{},"metadata":{"_buckets":{"deposit":"7201c187-060e-4327-807c-08b07bfcae7f"},"_deposit":{"created_by":74,"id":"2000882","owners":[74],"pid":{"revision_id":0,"type":"depid","value":"2000882"},"status":"published"},"_oai":{"id":"oai:jircas.repo.nii.ac.jp:02000882","sets":["1709882237727"]},"author_link":[],"control_number":"2000882","item_30002_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-07-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"55","bibliographicPageStart":"1"}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000121078171","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000121078171"}],"affiliationNames":[{"affiliationName":"Japan International Research Center for Agricultural Sciences","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Yamanaka, Naoki","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author","nameIdentifiers":[{"nameIdentifier":"0000-0002-5099-3157","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-5099-3157"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000121078171","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000121078171"}],"affiliationNames":[{"affiliationName":"Japan International Research Center for Agricultural Sciences","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kato, Masayasu","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author","nameIdentifiers":[{"nameIdentifier":"0000-0003-3642-1934","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-3642-1934"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000122220432","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000122220432"}],"affiliationNames":[{"affiliationName":"National Agriculture and Food Research Organization","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Akamatsu, Hajime","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000123694728","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000123694728"}],"affiliationNames":[{"affiliationName":"University of Tsukuba","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Yamaoka, Yuichi","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author","nameIdentifiers":[{"nameIdentifier":"0000-0002-0411-6110","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-0411-6110"}]}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Chapter 1: Evaluation of resistance to soybean rust Section\n1-1. Multiplication of rust urediniospores : 3\n1-2. Single-lesion isolation : 6\n1-2’. Single-spore isolation : 9\n1-3. Inoculation of soybean with spore suspension : 12\n1-3’. Inoculation of soybean with spore suspension (Leaf culture method) : 17\n1-4. Evaluation of rust pathogenicity : 18\n1-5. Rust resistance of soybean genotypes : 21\n1-6. Rust tolerance of soybean genotypes : 24\n1-7. Data analysis : 27\n\nChapter 2: Simple sequence repeat (SSR) marker analysis (Toward marker-assisted selection to introduce Rpp genes)\n2-1. Sampling for DNA extraction : 29\n2-2. DNA extraction : 32\n2-3. Adjustment of DNA concentration : 34\n2-4. PCR : 37\n2-5. Acrylamide gel electrophoresis : 39\n2-6. Data analysis : 43\n\nAcknowledgments : 50\nReferences : 52\nVersion info and authors : 55","subitem_description_language":"en","subitem_description_type":"TableOfContents"},{"subitem_description":"Version information-Publication date on the website\nVersion 27 (English and Spanish):  July 3, 2023","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-05-15"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2023-07-03"}],"filename":"Laboratory_manual_v.27.1.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"Laboratory_manual_v.27.1","objectType":"fulltext","url":"https://jircas.repo.nii.ac.jp/record/2000882/files/Laboratory_manual_v.27.1.pdf"},"version_id":"789f4c91-5af7-439f-a1fa-6f0fbc6a085e"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-05-19"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2023-07-03"}],"filename":"Laboratory_manual_V27.1_Thumbnail.jpg","filesize":[{"value":"28 KB"}],"format":"image/jpeg","mimetype":"image/jpeg","url":{"label":"Laboratory_manual_V27.1_Thumbnail","objectType":"thumbnail","url":"https://jircas.repo.nii.ac.jp/record/2000882/files/Laboratory_manual_V27.1_Thumbnail.jpg"},"version_id":"338a135e-0116-447a-a267-13579c986c9b"}]},"item_30002_funding_reference21":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"a1B1","subitem_award_uri":"https://www.jircas.go.jp/en/program/prob/b1"},"subitem_award_titles":[{"subitem_award_title":"Development of resilient crops and production technologies","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100009472","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Japan International Research Center for Agricultural Sciences (JIRCAS)","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"B4","subitem_award_uri":"https://www.jircas.go.jp/en/program/program_b/b4"},"subitem_award_titles":[{"subitem_award_title":"Development of Management Technologies against Migrating Pests and Diseases over Countries","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100009472","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Japan International Research Center for Agricultural Sciences (JIRCAS)","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"B3","subitem_award_uri":"https://www.jircas.go.jp/en/program/program_b/20161108"},"subitem_award_titles":[{"subitem_award_title":"Development of Breeding Technologies toward Improved Production and Stable Supply of Upland Crops","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100009472","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Japan International Research Center for Agricultural Sciences (JIRCAS)","subitem_funder_name_language":"en"}]}]},"item_30002_identifier_registration17":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.34556/0002000882","subitem_identifier_reg_type":"JaLC"}]},"item_30002_language12":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan International Research Center for Agricultural Sciences","subitem_publisher_language":"en"}]},"item_30002_relation18":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"es","subitem_relation_name_text":"Manual de laboratorio para estudios sobre la resistencia a roya de la soja"}],"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://jircas.repo.nii.ac.jp/records/2000881","subitem_relation_type_select":"URI"}}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_30002_rights6":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2013-2023 Japan International Research Center for Agricultural Sciences (JIRCAS)","subitem_rights_language":"en"},{"subitem_rights":"About the copyright and the notice with regard to use: The copyright of this manual belongs to Japan International Research Center for Agricultural Sciences (JIRCAS). This manual may not be modified or copied in whole or in part without the permission of JIRCAS (headquarters: 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan), except for brief excerpts in connection with reviews or academic activities. In case you make the research results based on this manual public via articles, meetings, print, Internet, etc., you must cite this manual and its URL. In addition, please contact us through this form. The experimental data in this manual were obtained by the methods in it, however, they may fluctuate according to the differences in cultivation conditions. Please be aware that the contents of the manual will be periodically updated and check the date and the version of the downloaded manual. In case you have any questions or comments regarding for this manual, please mail to us through this form.  Please download the files if you confirm and agree to the above conditions.","subitem_rights_language":"en","subitem_rights_resource":"https://www.jircas.go.jp/en/publication/manual_guideline/30"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Laboratory manual for studies on soybean rust resistance","subitem_title_language":"en"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"Laboratory manual for studies on soybean rust resistance","item_type_id":"40039","owner":"74","path":["1709882237727"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-05-13"},"publish_date":"2025-05-13","publish_status":"0","recid":"2000882","relation_version_is_last":true,"title":["Laboratory manual for studies on soybean rust resistance"],"weko_creator_id":"74","weko_shared_id":-1},"updated":"2025-05-22T05:50:43.554664+00:00"}