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Agrobacterium-mediated transformation system using immature embryos can effectively generate transgenic rice plants with a single copy of the transgene
https://doi.org/10.34556/0002000412
https://doi.org/10.34556/0002000412ccee37c8-7529-419e-885e-99ef230979ab
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
jircas_working_report91-_25-34 (668KB)
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| Item type | 国際農研デフォルトアイテムタイプ（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-07-25 | |||||||
| タイトル | ||||||||
| タイトル | Agrobacterium-mediated transformation system using immature embryos can effectively generate transgenic rice plants with a single copy of the transgene | |||||||
| 言語 | en | |||||||
| 作成者 |
Ishizaki, Takuma
× Ishizaki, Takuma
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 主題 | ||||||||
| 主題 | Copy number, Immature embryos, Rice, Transformation | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Agrobacterium-mediated transformation in rice has been widely used for both basic and applied research. Previous reports on Agrobacterium-mediated transformation in rice have used mature seed-derived callus or immature embryos as starting materials and have briefly mentioned that the transgenic plants generated by these methods contained a low copy number of the transgene, which offers less labor to fix transgene genetically, to evaluate position effects, and to determine insertion site of transgene, particularly in the transgenic plants with one copy of transgene. However, most of them did not include quantitative observations regarding copy numbers of the transgene; the relationship between transgene copy numbers and starting materials, seed-derived callus, or immature embryos, has also not been elucidated. In this study, we generated transgenic rice plants from mature seed-derived callus and immature embryos of the rice variety Nipponbare and then compared the copy numbers of the transgene in transgenic plants. Transgenic plants from immature embryos had a significantly lower copy number of the transgene than those obtained from mature seed-derived callus, and the percentage of transgenic plants with one copy of the transgene was also significantly different: 25.0% from mature seed-derived callus and 46.7% from immature embryos, respectively. We generated transgenic plants in other five rice varieties—Koshihikari, NERICA1, NERICA4, Curinga, and Kasalath—by transformation using immature embryos. Transgenic plants with one copy of the transgene were generated at a high frequency in all varieties examined; this frequency ranged from 37.6 to 87.0%. We conclude that Agrobacterium-mediated transformation using immature embryos effectively generates transgenic rice plants with one copy of the transgene—a desirable trait in molecular breeding and in basic plant research. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | Japan International Research Center for Agricultural Sciences | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_3248 | |||||||
| 資源タイプ | book part | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| ID登録 | ||||||||
| ID登録 | 10.34556/0002000412 | |||||||
| ID登録タイプ | JaLC | |||||||
| 関連情報 | ||||||||
| 関連タイプ | isPartOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.34556/0002000404 | |||||||
| 関連名称 | Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 1341-710X | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA11159468 | |||||||
| 書誌情報 |
en : JIRCAS Working Report : Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions 巻 91, p. 25-34, 発行日 2020-08-20 |
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| 助成情報 | ||||||||
| 識別子タイプ | Crossref Funder | |||||||
| 助成機関識別子 | https://doi.org/10.13039/501100003993 | |||||||
| 助成機関名 | Ministry of Agriculture, Forestry and Fisheries, Japan (MAFF)(en) | |||||||
| 研究課題名 | Development of Abiotic Stress Tolerant Crops by DREB Genes; Development of Drought-tolerant Crops for Developing Countries(en) | |||||||
