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Sustainable PHB production by type II methanotroph Methylocystis sp. NK4-5–1 utilizing oil palm sap-derived biogas
https://jircas.repo.nii.ac.jp/records/2001054
https://jircas.repo.nii.ac.jp/records/20010548fd2d610-5f17-4cbc-bf2c-701705a430f7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1-s2.0-S2352186425005929-main (6.2 MB)
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| Item type | 国際農研デフォルトアイテムタイプ（フル）(1) | |||||||||||||||||||||
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| 公開日 | 2025-11-26 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | Sustainable PHB production by type II methanotroph Methylocystis sp. NK4-5–1 utilizing oil palm sap-derived biogas | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 作成者 |
Arai, Takamitsu
× Arai, Takamitsu
× Aikawa, Shimpei
ORCID
0000-0001-9202-0225
× Sudesh, Kumar
ORCID
0000-0003-4756-9192
× Fazita, MR Nurul
ORCID
0000-0001-9979-2295
× Leh, Cheu Peng
ORCID
0000-0002-8232-7781
× Haafiz, MK Mohamad
ORCID
0000-0002-4344-0554
× Tay, Guan Seng
ORCID
0000-0002-7352-023X
× Kosugi, Akihiko
ORCID
0000-0002-3130-1151
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| アクセス権 | ||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||
| 権利情報 | © 2025 The Authors. Published by Elsevier B.V. | |||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||
| 権利情報Resource | https://creativecommons.org/licenses/by/4.0/deed.en | |||||||||||||||||||||
| 権利情報 | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | |||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||
| 権利情報 | https://creativecommons.org/licenses/by/4.0/ | |||||||||||||||||||||
| 主題 | ||||||||||||||||||||||
| 主題 | Simple two-step enrichment, Type II Methanotrophs, Nutrient, Polyhydroxybutyrate, Biogas valorization, Oil palm sap | |||||||||||||||||||||
| 内容記述 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | Biopolymers derived from biogas offer a sustainable approach to mitigating plastic pollution and greenhouse gas emissions. This study focused on the enrichment and isolation of polyhydroxybutyrate (PHB)-producing methanotrophs using a simple two-step dilution method, and evaluated PHB production from biogas generated from oil palm sap (OPS). Amplicon sequencing post-enrichment identified a microbial community dominated by Methylophilaceae (42.3%) and Methylocystis (41.5%). Methylocystis sp. NK4–5–1 was successfully isolated and demonstrated PHB synthesis from synthetic methane. The strain preferred ammonium sulfate over potassium nitrate as a nitrogen source at 0.1g N/L, with optimum conditions at 25℃ and pH 6.5. Under optimized conditions with synthetic methane, the strain achieved a maximum PHB content of 29% ± 3.8% (w/w) within 48 h. Moreover, the cultivation of Methylocystis sp. NK4–5–1 on OPS derived biogas under fed-batch conditions with a final methane concentration of 30% yielded a cell dry weight of 0.59 ± 0.06 g/L and a PHB content of 13.1 ± 3.8% (w/w) after 10 days, confirming effective biogas valorization. ¹H NMR analysis verified the structural identity of PHB from both substrates. Notably, biogas-derived PHB exhibited higher molecular weight and melting temperature (Mw = 536kDa; Tm = 176℃) than that from synthetic methane (Mw=348kDa; Tm = 170℃), indicating improved thermal stability and material performance. These findings demonstrate the potential of biogas valorization for sustainable bioplastic production and provide practical criteria for strain selection and process optimization. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||||||||
| 日付 | ||||||||||||||||||||||
| 日付 | 2025-10-28 | |||||||||||||||||||||
| 日付タイプ | Accepted | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
| 関連情報 | ||||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.eti.2025.104606 | |||||||||||||||||||||
| 収録物識別子 | ||||||||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||||||||
| 収録物識別子 | 2352-1864 | |||||||||||||||||||||
| 書誌情報 |
en : Environmental Technology & Innovation 巻 40, p. 104606, 発行日 2025-11-05 |
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| 助成情報 | ||||||||||||||||||||||
| 識別子タイプ | Crossref Funder | |||||||||||||||||||||
| 助成機関識別子 | https://doi.org/10.13039/501100009472 | |||||||||||||||||||||
| 助成機関名 | Japan International Research Center for Agricultural Sciences (JIRCAS)(en) | |||||||||||||||||||||
| 研究課題番号URI | https://www.jircas.go.jp/en/program/proa/a2 | |||||||||||||||||||||
| 研究課題番号 | 05a1A2 | |||||||||||||||||||||
| 研究課題名 | Development of carbon recycling technologies to address global issues caused by agricultural waste(en) | |||||||||||||||||||||
