{"created":"2025-07-23T00:51:53.316777+00:00","id":2000892,"links":{},"metadata":{"_buckets":{"deposit":"2dfd6352-752b-4940-8858-2cb4affb0885"},"_deposit":{"created_by":74,"id":"2000892","owners":[74],"pid":{"revision_id":0,"type":"depid","value":"2000892"},"status":"published"},"_oai":{"id":"oai:jircas.repo.nii.ac.jp:02000892","sets":["1718256554766:1718256617194"]},"author_link":[],"control_number":"2000892","item_30002_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-07-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"115","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"Marine Biotechnology","bibliographic_titleLang":"en"}]}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000121078171","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000121078171"}],"affiliationNames":[{"affiliationName":"Japan International Research Center for Agricultural Sciences (JIRCAS)","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Matsuda, Ryuya","creatorNameLang":"en","creatorNameType":"Personal"}],"creatorType":"Author","nameIdentifiers":[{"nameIdentifier":"0000-0002-7213-453X","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-7213-453X"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000089022273","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000089022273"}],"affiliationNames":[{"affiliationName":"Nagasaki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kuwano, Kazuyoshi","creatorNameLang":"en"}]}]},"item_30002_date11":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2025-07-11","subitem_date_issued_type":"Accepted"}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Asparagopsis taxiformis is a seaweed of interest for use as a livestock feed ingredient because of its high bromoform content, effectively reducing methane emissions from ruminants. A reliable protocol covering hatchery and nursery phases is essential to develop aquaculture techniques for gametophytes. The aim of this study was to establish a seedling production method for gametophytes under controlled laboratory conditions. We conducted the following: (1) induction of tetraspore release, (2) assessment of juvenile gametophyte growth, and (3) evaluation of gametophyte quality through bromoform quantification. Tetraspore release was successfully induced at 25 ℃ under an equinox photoperiod (12 h light: 12 h dark) or at 20 ℃ under short photoperiod (8 h light: 16 h dark), with initial spore release occurring after 12.3 ± 1.03 and 15.1 ± 1.26 days, respectively. Germinated tetraspores developed into 3–5 cm gametophytes over 2 months through static culture, followed by an aeration phase using natural seawater. Artificial seawater failed to promote gametophyte growth. Aeration improved the daily growth rate (6.86 ± 0.36%) compared to that of the static culture (4.72 ± 0.51%). Further cultivation promoted the development of main axes, lateral branches, and rhizomes. Bromoform was predominantly accumulated in the lateral branches (17.8 ± 12.3 mg·g⁻¹ dry weight (DW)) and rhizomes (12.2 ± 1.37 mg·g⁻¹ DW), compared to main axes (5.27 ± 1.95 mg·g⁻¹ DW). Unlike tetrasporophytes, which constantly released bromoforms into the medium, gametophytes maintained stable extracellular bromoform levels. These findings demonstrate a feasible approach for laboratory-scale production of A. taxiformis gametophytes with consistent bromoform accumulation, contributing to the advancement of its aquaculture.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-07-23"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2025-07-21"}],"filename":"2000892.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"label":"2000892","objectType":"fulltext","url":"https://jircas.repo.nii.ac.jp/record/2000892/files/2000892.pdf"},"version_id":"06ffccfd-17fc-4e2b-aa2b-b896e696e0cf"}]},"item_30002_funding_reference21":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"05a1B4","subitem_award_uri":"https://www.jircas.go.jp/en/program/prob/b4"},"subitem_award_titles":[{"subitem_award_title":"Development and dissemination of sustainable aquaculture technologies in the tropical area based on the eco-system approach","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100009472","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Japan International Research Center for Agricultural Sciences (JIRCAS)","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"25K09257","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25K09257/"},"subitem_award_titles":[{"subitem_award_title":"紅藻カギケノリのブロモホルム合成機構の解明 : その養殖は他の海藻と共存できるか？","subitem_award_title_language":"ja"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Japan Society for the Promotion of Science (JSPS)","subitem_funder_name_language":"en"}]}]},"item_30002_language12":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer","subitem_publisher_language":"en"}]},"item_30002_relation18":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s10126-025-10493-2","subitem_relation_type_select":"DOI"}}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_30002_rights6":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2025","subitem_rights_language":"en"},{"subitem_rights":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit  http://creativecommons.org/licenses/by/4.0/.","subitem_rights_language":"en","subitem_rights_resource":"http://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"The version of record of this article, first published in Marine Biotechnology is available online at Publisher’s website: https://doi.org/10.1007/s10126-025-10493-2","subitem_rights_language":"en"}]},"item_30002_source_identifier22":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"1436-2236","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"1436-2228","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA11628864","subitem_source_identifier_type":"NCID"}]},"item_30002_subject8":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"Seaweed aquaculture","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Seedling production","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Life cycle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tetrasporogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Halogen metabolism","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Demonstration of Bromoform-Producing  Gametophyte Culture for a Red Alga, Asparagopsis taxiformis in Laboratory Conditions","subitem_title_language":"en"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"A Demonstration of Bromoform-Producing  Gametophyte Culture for a Red Alga, Asparagopsis taxiformis in Laboratory Conditions","item_type_id":"40039","owner":"74","path":["1718256617194"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-07-23"},"publish_date":"2025-07-23","publish_status":"0","recid":"2000892","relation_version_is_last":true,"title":["A Demonstration of Bromoform-Producing  Gametophyte Culture for a Red Alga, Asparagopsis taxiformis in Laboratory Conditions"],"weko_creator_id":"74","weko_shared_id":-1},"updated":"2025-08-18T04:48:55.284187+00:00"}