{"created":"2024-07-25T01:46:51.262635+00:00","id":2000416,"links":{},"metadata":{"_buckets":{"deposit":"d3434e13-0e48-4e26-98ac-bafc32df8164"},"_deposit":{"created_by":74,"id":"2000416","owner":"74","owners":[74],"pid":{"revision_id":0,"type":"depid","value":"2000416"},"status":"published"},"_oai":{"id":"oai:jircas.repo.nii.ac.jp:02000416","sets":["1698892316070:1721355674911"]},"author_link":[],"control_number":"2000416","item_30002_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-08-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"88","bibliographicPageStart":"57","bibliographicVolumeNumber":"91","bibliographic_titles":[{"bibliographic_title":"JIRCAS Working Report : Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions","bibliographic_titleLang":"en"}]}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fuganti-Pagliarini, Renata","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Rockenbach Marin, Silvana Regina","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Côrrea Molinari, Mayla Daiane","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"De Amorim Barbosa, Daniel","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Molinari, Hugo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mertz-Henning, Liliane Márcia","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Neumaier, Norman","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Bouças Farias, José Renato","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakashima, Kazuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamaguchi-Shinozaki, Kazuko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Lima Nepomuceno, Alexandre","creatorNameLang":"en"}]}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Drought is one of the greatest sources of environmental stress that has resulted in both economic and yield losses in many soybean-producing regions. The use of biotechnological tools aimed to produce plants with increased drought tolerance and productivity is the result of major investments in scientific and technological research. During the last decades, transcription factors (TFs) and key genes of important drought-responsive pathways have been used to develop genetically modified (GM) plants with increased tolerance to abiotic stress. To develop soybean lines with improved drought tolerance, genes encoding dehydration-responsive element binding protein (DREB) TFs and ABA-responsive element-binding proteins (AREB) as well as the GolS and NCED genes that encode the enzyme galactinol synthase (GolS, EC 2.4.1.123) of the raffinose family of oligosaccharides (RFOs) and the key enzyme in abscisic acid (ABA) biosynthesis 9-cisepoxycarotenoid dioxygenase (NCED, EC 1.13.11.51), respectively, were successfully introduced in soybean plants. After transformation, it was imperative to characterize the resultant drought tolerance of the GM lines. Thus, the soybean GM lines containing genetic constructions to overexpress AtDREB1A, GmDREB1A, AtDREB2A, AtAREB1, AtGolS2, and AtNCED3 were phenotyped based on molecular and agronomical traits, growth parameters, and survival rates under water deficit conditions in experiments carried out under greenhouse and field conditions for more than one crop season. All obtained results were compiled and are presented in this report. Overall, the GM lines are promising and show improved drought tolerance as diverse defense mechanisms aimed at surviving periods of water scarcity were targeted while retaining the productivity of the crop. These outcomes highlight soybean drought-tolerance pathways and indicate that the use of biotechnological tools in agricultural research can help producers minimize both yield and financial losses during drought-stricken crop seasons.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-07-25"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2020-08-20"}],"filename":"jircas_working_report91-_57-88.pdf","filesize":[{"value":"1.13MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"jircas_working_report91-_57-88","objectType":"fulltext","url":"https://jircas.repo.nii.ac.jp/record/2000416/files/jircas_working_report91-_57-88.pdf"},"version_id":"5716a0af-233b-49f9-8f5c-b5a3e7286ad0"}]},"item_30002_funding_reference21":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"JPMJSA0907","subitem_award_uri":"https://doi.org/10.52926/JPMJSA0907"},"subitem_award_titles":[{"subitem_award_title":"Science and Technology Research Partnership for Sustainable Development (SATREPS) : Development of genetic engineering technology of crops with stress tolerance against degradation of global environment","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100002241","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"Japan Science and Technology Agency (JST)","subitem_funder_name_language":"en"}]}]},"item_30002_identifier_registration17":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.34556/0002000416","subitem_identifier_reg_type":"JaLC"}]},"item_30002_language12":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan International Research Center for Agricultural Sciences","subitem_publisher_language":"en"}]},"item_30002_relation18":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions"}],"subitem_relation_type":"isPartOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.34556/0002000404","subitem_relation_type_select":"DOI"}}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"book part","resourceuri":"http://purl.org/coar/resource_type/c_3248"}]},"item_30002_source_identifier22":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"1341-710X","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA11159468","subitem_source_identifier_type":"NCID"}]},"item_30002_subject8":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"Glycine max","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transcription factor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DREB","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"AREB","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ABA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"galactinol synthase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"9-cis-epoxycarotenoid dioxygenase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"water deficit, yield","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"yield","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Drought-tolerant soybean development: evaluation of GM lines under greenhouse and field conditions","subitem_title_language":"en"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"Drought-tolerant soybean development: evaluation of GM lines under greenhouse and field conditions","item_type_id":"40039","owner":"74","path":["1721355674911"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-07-25"},"publish_date":"2024-07-25","publish_status":"0","recid":"2000416","relation_version_is_last":true,"title":["Drought-tolerant soybean development: evaluation of GM lines under greenhouse and field conditions"],"weko_creator_id":"74","weko_shared_id":-1},"updated":"2025-04-04T04:09:57.797119+00:00"}