{"created":"2024-02-13T06:07:27.905461+00:00","id":2000118,"links":{},"metadata":{"_buckets":{"deposit":"75ccce6a-f101-4d08-8e8f-fe726f302f92"},"_deposit":{"created_by":8,"id":"2000118","owner":"8","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2000118"},"status":"published"},"_oai":{"id":"oai:jircas.repo.nii.ac.jp:02000118","sets":["1704421711574:1705364319042"]},"author_link":[],"control_number":"2000118","item_30002_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"JIRCAS Journal","bibliographic_titleLang":"en"}]}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKASHIMA, Kazuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"PROMMINTARA, Maitree","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"OHTSU, Yoshihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"KANO, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"IMADA, Jun","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"KOIZUMI, Meisaku","creatorNameLang":"en"}]}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citrus greening disease caused by bacterium-like organisms (BLO; greening organisms: GOs) is one of the most destructive diseases of citrus in Thailand. An efficient and reliable detection method of the pathogen is urgently needed for the control of the disease. Recently, polymerase chain reaction (PCR) method for amplifying the 16S rDNA fragment of the GOs has been developed by Jagoueix et al. (1994; Int. J. Syst. Bacteriol. 44: 379-386). We applied the method for the detection of Thai GO isolates, Nakorn-Pathum, Rangsit and Nan isolates, and succeeded in detecting the 16S rDNA fragments of the Thai isolates. Sequence analysis of the amplified 16S rDNA fragment of the Nakorn-Pathum isolate revealed that the isolate is very close to the Indian isolate of liberobacter phylogenetically. By simplifying the DNA extraction procedure, we were able to obtain DNA for PCR within 20 min, and detect the GO within 4.5 hr.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"バクテリア様微生物(BLO; グリーニング病病原体: GO)により引き起こされるカンキツグリーニング病は，熱帯地域のカンキツ類において，最も被害が大きい病気である。本病を防除するためには，感染樹の早期検定・除去が必要であり，迅速で信頼性の高い病原体検出法の開発が必要とされていた。最近，Jagoueixら(1994; Int. J. Syst. Bacteriol. 44:379-386)によって，PCR法によりGOの16S rDNAを増幅する方法が開発された。我々は，このPCR法の適用により，GOのタイ株であるNakorn Pathum株，Rangsit株，Nan株の16S rDNA断片を検出することに成功した。Nakorn Pathum株の16S rDNA断片の塩基配列を解析した結果，本株はliberobacterインド株と，系統進化的に極めて近いことが明らかになった。PCR用のDNAの抽出法を簡易化することにより，4.5時間以内にGOを検出することが可能になった。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-02-13"}],"filename":"jircas_journal3-_1-8.pdf","filesize":[{"value":"526 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"jircas_journal3-_1-8","objectType":"fulltext","url":"https://jircas.repo.nii.ac.jp/record/2000118/files/jircas_journal3-_1-8.pdf"},"version_id":"02aa6b1c-7364-494e-82b6-0a9eb28e4c3b"}]},"item_30002_identifier_registration17":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.34556/0002000118","subitem_identifier_reg_type":"JaLC"}]},"item_30002_language12":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan International Research Center for Agricultural Sciences","subitem_publisher_language":"en"}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_30002_source_identifier22":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"13407686","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA11030483","subitem_source_identifier_type":"NCID"}]},"item_30002_subject8":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"citrus greening","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liberobacter","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"16S rDNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rapid diagnosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of 16S rDNA of Thai Isolates of Bacterium-Like Organisms Associated with Greening Disease of Citrus","subitem_title_language":"en"},{"subitem_title":"カンキツグリーニング病の病原バクテリア様微生物（タイ株）の 16S rDNAの検出","subitem_title_language":"ja"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"Detection of 16S rDNA of Thai Isolates of Bacterium-Like Organisms Associated with Greening Disease of Citrus","item_type_id":"40039","owner":"8","path":["1705364319042"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-02-13"},"publish_date":"2024-02-13","publish_status":"0","recid":"2000118","relation_version_is_last":true,"title":["Detection of 16S rDNA of Thai Isolates of Bacterium-Like Organisms Associated with Greening Disease of Citrus"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2024-02-14T01:26:13.383618+00:00"}