{"created":"2024-02-07T05:41:06.619988+00:00","id":2000113,"links":{},"metadata":{"_buckets":{"deposit":"b93176da-6cbe-47d5-91c8-f6f4a61e1bcf"},"_deposit":{"created_by":8,"id":"2000113","owner":"8","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2000113"},"status":"published"},"_oai":{"id":"oai:jircas.repo.nii.ac.jp:02000113","sets":["1704421711574:1705364280817"]},"author_link":[],"control_number":"2000113","item_30002_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-09-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"57","bibliographicPageStart":"49","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"JIRCAS Journal","bibliographic_titleLang":"en"}]}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OKADA, Noriyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"CHETTANACHITARA, Charan","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"DAENGSUBHA, Wiwut","creatorNameLang":"en"}]}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"From Tua-Nao, a non-salt fermented soybean food of Thailand, bacteria which can be utilized for the improvement of Natto bacterium by cell fusion technique were screened. Screening was carried out for vitamin B<sub>12</sub>-producing bacteria which are heat-resistant and lysozyme-sensitive. Tua-Nao samples were collected at Lam Phun, Phrao, Doi Saket and Mae Hong Son in northern Thailand. Bacterial counts in Tua-Nao samples were markedly different in number and the ratio of the number of heat-resistant bacteria to the number of total bacteria was also different. Vitamin B<sub>12</sub> content varied and some samples did not contain vitamin B<sub>12</sub> at all. The lysozyme sensitivities and vitamin B<sub>12</sub>-producing ability of bacteria separated from Tua-Nao samples after heating were examined. Among 120 isolates, about 20 were lysozyme-sensitive and one (TN02-13) produced vitamin B<sub>12</sub>. However the isolate (TN02-13) was a mixture of two bacterial isolates (TN02-13a and TN02-13b) and TN02-13b was found to produce vitamin B<sub>12</sub> and to be lysozyme-sensitive. Thus a vitamin B<sub>12</sub>-producing bacterium was obtained from Tua-Nao. This bacterium was regenerated from a protoplast form into a bacillary form.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"タイの無塩大豆発醗酵食品より，細胞融合による納豆菌の改良に利用可能なバクテリアを探索した。検索は，リゾチーム感受性かつ耐熱性のビタミンB<sub>12</sub>生産菌を求めて行った。トゥアナオ試料は北タイのラムプーン，プラオ，ドイサケット，メーホーンソンより採取した。トゥアナトのバクテリア数は試料によりかなり異なった。耐熱性／非耐熱性の比も大きく異なった。またトゥアナオはビタミンB<sub>12</sub>含量が異なるのみでなく，含まないものもあった。ビタミンB<sub>12</sub>を含むトゥアナオ試料より，熱処理後バクテリアを分離し，リゾチーム感受性，ビタミンB<sub>12</sub>生産能を調べた。分離株120株のうち，リゾチーム感受性を示す約20株を得，そのうちビタミンB<sub>12</sub>生産能を有する1株(TN02-13)を見いだした。本菌は混合菌(TN02-13a,TN02-13b)であったが，再分離後TN02-13bにリゾチーム感受性かつビタミンB<sub>12</sub>生産能があることが分かった。また本菌はプロトプラストから桿菌へ再生した。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-02-07"}],"filename":"jircas_journal2-_49-57.pdf","filesize":[{"value":"433 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"jircas_journal2-_49-57","objectType":"fulltext","url":"https://jircas.repo.nii.ac.jp/record/2000113/files/jircas_journal2-_49-57.pdf"},"version_id":"eab59a14-0d31-4cc3-b1a2-545f51ee22c0"}]},"item_30002_identifier_registration17":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.34556/0002000113","subitem_identifier_reg_type":"JaLC"}]},"item_30002_language12":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan International Research Center for Agricultural Sciences","subitem_publisher_language":"en"}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_30002_source_identifier22":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"13407686","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA11030483","subitem_source_identifier_type":"NCID"}]},"item_30002_subject8":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"fermented soybeans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Natto","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell fusion","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Screening of Bacteria Producing Vitamin B<sub>12</sub> from Tua-Nao in Thailand","subitem_title_language":"en"},{"subitem_title":"タイのトゥアナオよりビタミンB<sub>12</sub>生産菌の分離","subitem_title_language":"ja"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"Screening of Bacteria Producing Vitamin B<sub>12</sub> from Tua-Nao in Thailand","item_type_id":"40039","owner":"8","path":["1705364280817"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-02-07"},"publish_date":"2024-02-07","publish_status":"0","recid":"2000113","relation_version_is_last":true,"title":["Screening of Bacteria Producing Vitamin B<sub>12</sub> from Tua-Nao in Thailand"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2024-05-28T06:08:20.761695+00:00"}