{"created":"2024-02-02T04:25:36.944967+00:00","id":2000105,"links":{},"metadata":{"_buckets":{"deposit":"746af857-cee8-4722-b973-0d5ed558fc3f"},"_deposit":{"created_by":8,"id":"2000105","owner":"8","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2000105"},"status":"published"},"_oai":{"id":"oai:jircas.repo.nii.ac.jp:02000105","sets":["1704421711574:1705363447547"]},"author_link":[],"control_number":"2000105","item_30002_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-10-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"67","bibliographicPageStart":"57","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"JIRCAS Journal","bibliographic_titleLang":"en"}]}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKASHIMA,  Kazuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"CHALEEPROM, Witcha","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"WONGKAEW, Porntip","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"SIRITHORN, Pisan","creatorNameLang":"en"}]}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Sugarcane white leaf disease (SCWL) is one of the most destructive diseases of sugarcane in Thailand. An efficient and reliable method to detect the pathogen is urgently needed for the development of methods of control of the disease. We isolated the DNA of the causal agent, a mycoplasma-like organism (MLO), cloned random DNA fragments, and used them as DNA probes to detect the pathogen rapidly and easily. DNA of the SCWL MLO was isolated from the host DNA by repeated bisbenzimide-CsCI equilibrium density gradient centrifugation. The DNA fragments cut by restriction enzyme HindIII were cloned in a plasmid-Escherichia coli system. Southern hybridization analysis revealed that the inserts of some recombinant plasmids consisted of fragments of chromosomal DNA of the MLO, whereas the inserts of the other recombinant plasmids consisted of fragments of extrachromosomal DNA of the MLO. Cloned DNA probes were successfully applied in dot hybridization to detect the MLO in sugarcane plants and in the insect vector Matsumuratettix hiroglyphicus. Dot hybridization using the DNA probes indicated that SCWL MLO and MLOs of gramineous plants including rice yellow dwarf MLO shared a greater nucleotide sequence homology with one another than with other MLOs. The method developed was found to be useful to detect the pathogen infecting sugarcane plants in the fields of the Khon Kaen area in Thailand.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"サトウキビ白葉病(SCWL)は，タイのサトウキビにとって最も重要な病害である。本病の病原体を検出するための，有効で信頼性の高い方法が，強く求められていた。我々は，本病の病原体であるマイコプラズマ様微生物(MLO)のDNAを分離し，そのランダムなDNAをクローニングして，DNAプローブとして用いることにより，病原体を迅速かつ簡便に検出することができた。SCWL MLOのDNAは，ビスベンズイミド―塩化セシウム平衡密度勾配遠心を繰り返すことにより，宿主のDNAから分離された。制限酵素HindIIIで切断して得られたDNA断片は，プラスミド―大腸菌の系にクローニングされた。サザンハイブダイゼーションにより，いくつかの組み換えプラスミド挿入断片は，MLOの染色体断片からなり，他の組み換えプラスミド挿入断片は，MLOの染色体外DNA断片からなることが明らかになった。クローン化したDNAプローブを用いたドットハイブリダイゼーションにより，サトウキビ及び媒介昆虫Matsumuratettix hiroglyphicusからMLOを検出することができた。これらのDNAプローブを用いたドットハイブルダイゼーションにより，SCWL MLOは，イネ黄萎病MLOを含むイネ科植物のMLOと塩基配列の相同性は高いが，他の種類のMLOとの相同性は低いことが示唆された。本検出法をタイ国コンケン地区のSCWL MLOの検出に適用し，極少量の植物組織からの病原体検出において，極めて優れていることを確認した。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-02-02"}],"filename":"jircas_journal1-_57-67.pdf","filesize":[{"value":"709 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"jircas_journal1-_57-67","objectType":"fulltext","url":"https://jircas.repo.nii.ac.jp/record/2000105/files/jircas_journal1-_57-67.pdf"},"version_id":"b876baad-d5f0-46cb-b0b0-ed4cafc18abb"}]},"item_30002_identifier_registration17":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.34556/0002000105","subitem_identifier_reg_type":"JaLC"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan International Research Center for Agricultural Sciences","subitem_publisher_language":"en"}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_30002_source_identifier22":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"13407686","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AA11030483","subitem_source_identifier_type":"NCID"}]},"item_30002_subject8":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"MLO","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dot hybridization","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of Mycoplasma-like Organisms Associated with White Leaf Disease of Sugarcane in Thailand using DNA Probes","subitem_title_language":"en"},{"subitem_title":"DNAプローブによるタイのサトウキビ白葉病病原マイコプラズマ様微生物の検出","subitem_title_language":"ja"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"Detection of Mycoplasma-like Organisms Associated with White Leaf Disease of Sugarcane in Thailand using DNA Probes","item_type_id":"40039","owner":"8","path":["1705363447547"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-02-02"},"publish_date":"2024-02-02","publish_status":"0","recid":"2000105","relation_version_is_last":true,"title":["Detection of Mycoplasma-like Organisms Associated with White Leaf Disease of Sugarcane in Thailand using DNA Probes"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2024-02-05T02:57:30.097313+00:00"}