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Laboratory manual for studies on soybean rust resistance Yamanaka, Naoki 0000-0002-5099-3157 Kato, Masayasu 0000-0003-3642-1934 Akamatsu, Hajime Yamaoka, Yuichi 0000-0002-0411-6110 Chapter 1: Evaluation of resistance to soybean rust Section 1-1. Multiplication of rust urediniospores : 3 1-2. Single-lesion isolation : 6 1-2’. Single-spore isolation : 9 1-3. Inoculation of soybean with spore suspension : 12 1-3’. Inoculation of soybean with spore suspension (Leaf culture method) : 17 1-4. Evaluation of rust pathogenicity : 18 1-5. Rust resistance of soybean genotypes : 21 1-6. Rust tolerance of soybean genotypes : 24 1-7. Data analysis : 27 Chapter 2: Simple sequence repeat (SSR) marker analysis (Toward marker-assisted selection to introduce Rpp genes) 2-1. Sampling for DNA extraction : 29 2-2. DNA extraction : 32 2-3. Adjustment of DNA concentration : 34 2-4. PCR : 37 2-5. Acrylamide gel electrophoresis : 39 2-6. Data analysis : 43 Acknowledgments : 50 References : 52 Version info and authors : 55 Version information-Publication date on the website Version 27 (English and Spanish): July 3, 2023 technical report Japan International Research Center for Agricultural Sciences 2023-07-03 VoR application/pdf image/jpeg 1 55 https://jircas.repo.nii.ac.jp/record/2000882/files/Laboratory_manual_v.27.1.pdf https://jircas.repo.nii.ac.jp/record/2000882/files/Laboratory_manual_V27.1_Thumbnail.jpg https://doi.org/10.34556/0002000882 https://jircas.repo.nii.ac.jp/records/2000882 eng Manual de laboratorio para estudios sobre la resistencia a roya de la soja https://jircas.repo.nii.ac.jp/records/2000881 open access © 2013-2023 Japan International Research Center for Agricultural Sciences (JIRCAS) About the copyright and the notice with regard to use: The copyright of this manual belongs to Japan International Research Center for Agricultural Sciences (JIRCAS). This manual may not be modified or copied in whole or in part without the permission of JIRCAS (headquarters: 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan), except for brief excerpts in connection with reviews or academic activities. In case you make the research results based on this manual public via articles, meetings, print, Internet, etc., you must cite this manual and its URL. In addition, please contact us through this form. The experimental data in this manual were obtained by the methods in it, however, they may fluctuate according to the differences in cultivation conditions. Please be aware that the contents of the manual will be periodically updated and check the date and the version of the downloaded manual. In case you have any questions or comments regarding for this manual, please mail to us through this form. Please download the files if you confirm and agree to the above conditions.